Protein science and quality

Watch or listen to Catherine discuss the company’s innovations for the market, and the wider challenges for the sector on the latest episode of The Future of Protein Production podcast.

There has been an impressive drive towards innovation in stem cell protocols this year. As 2021 comes to a close, we reflect on the highlights for stem cell science over the year. It was great to see new culture media, such as B8 and “Beefy-9”, published, developments in cultivated meat science hit the news and iPSC-based therapies edge closer to the clinic. Reproducibility remains the underlying theme, and indeed requirement, for maximising the impact of these wonderful developments and at Qkine we’ll continue to do everything we can to help in 2022.

Qkine and Enantis have concluded a global licensing deal for Enantis’s patented FGF2-STAB® technology. FGF2-STAB® is a hyperstable form of FGF-2 (bFGF), a core reagent for stem cell culture. FGF2-STAB (FGF2-G3) does not degrade in culture media for enhanced weekend-free stem cell culture media and advanced chemically defined systems, such as those needed in the cultured meat industry.

There is no fundamental difference between ESGRO and recombinant mouse leukaemia inhibitor factor (mLIF) protein. Both can be used for the successful maintenance of mouse embryonic stem cell (ESC) pluripotency. 10E7 units of ESGRO is equivalent to 100 µg of pure protein. Thank you to Susanne van den Brink (postdoc in Anna Bigas lab, IMIM, Barcelona) and David Turner (University of Liverpool) for sharing their resuspension protocol to prepare ESLIF media.

This Qkine post page covers “pioneering proteins launch” covering pioneering proteins launch, GMP recombinant proteins, animal-origin-free growth factors, cytokines for…

Video: Growth factor purity and its implications for stem cell culture Presentation by Luana Ferrara, Senior Protein Scientist Video script Slide 1: Introduction …

As a structural biologist, one will very quickly realise that protein not only needs to be pure, but it needs to be also homogeneous and the real proof of the protein’s quality is in its specific bioactivity. So,we clearly need different criteria for defining quality and that is what we do at Qkine.