Technote | TGF-ß (Qk010-CTGQk010)
Reconstituted animal origin-free TGF-β1 PLUS™ has excellent stability under standard storage conditions
Transforming growth factor beta-1 (TGF-β1) is a common component of human pluripotency maintenance media. However, as TGF-β1 is a highly complex protein, it is difficult to manufacture in microbial expression systems.
TGF-β1 PLUS™ is the world’s first animal origin-free TGF-β1, manufactured for highly reproducible results.
Qkine TGF-β1 one month stability
- TGF-β1 diluted to 50 µg/ml and stored in PBS + 0.1% BSA at -20 °C for one month had an EC50 of 0.070 ng/ml, equivalent to freshly reconstituted protein stored lyophilized at -80 °C, which had an EC50 of 0.050 ng/ml.
- When the 50 µg/ml TGF-β1 in PBS + 0.1% BSA was stored at 4 °C for one month it had an EC50 of 0.049 ng/ml, equivalent to the freshly reconstituted protein, which had an EC50 of 0.040 ng/ml.
- When diluted further to 1 µg/ml in cell culture medium and stored at 4 °C for one month TGF-β1 retained an EC50 of 0.043 ng/ml, equivalent to freshly reconstituted protein, which had an EC50 of 0.035 ng/ml.
TGF-β1 remained bioactive under all storage conditions. Bioactivity was determined using a CAGA reporter luciferase assay; transfected HEK293T cells were treated in triplicate with a serial dilution of TGF-β1 (Qk010) which had been reconstituted and stored under the conditions indicated. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity. (A) Bioactivity of control TGF-β1 (freshly reconstituted and diluted from protein stored lyophilized at -80 °C) compared with protein stored in PBS + 0.1% BSA at -20 °C for one month. (B) Bioactivity of a control protein compared with protein stored in PBS + 0.1% BSA at 4 °C for one month. (C) Bioactivity of control protein compared with protein stored in cell culture medium at 4 °C for one month.
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
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