Technote | TGF-ß (Qk010-CTGQk010)
Lyophilized TGF-β1 PLUS™ remains bioactive, stable and sterile after > 2 years storage in the freezer
Transforming growth factor beta-1 (TGF-β1) is a common component of human pluripotency maintenance media. However, as TGF-β1 is a highly complex protein, it is difficult to manufacture in microbial expression systems.
TGF-β1 PLUS™ is the world’s first animal origin-free TGF-β1, manufactured for highly reproducible results.
Qkine TGF-β1 PLUS™ > 2 years stability
- TGF-β1 PLUS™ stored at -80 °C for > 2 years (EC50 29.0 pg/ml) showed no difference in bioactivity compared to the newest available batch (34.5 pg/ml).
- SDS-PAGE showed no degradation of TGF-β1 PLUS™ was visible after > 2 years.
- Sterility tested showed no contamination after storage at -80 °C for > 2 years.
TGF-β1 PLUS™ remained stable and bioactive after > 2 years storage in the freezer. (A) Bioactivity was determined using a TGF-β1-responsive firefly luciferase reporter assay; transfected HEK293T cells were treated in triplicate with a serial dilution of TGF-β1 PLUS™ (Qk010) stored at -80 °C for > 2 years (green, > 2 years) or from the newest available batch (black, Control). Firefly luciferase activity was measured and normalized to control Renilla luciferase activity. (B) Lyophilized protein was stored at -80 °C for > 2 years. 3 µg was resolved on a 15% w/v SDS-PAGE gel under non-reduced conditions and stained with Coomassie Brilliant Blue R250. Data from Qk010 lot #204512 (test) and #204762 (control).
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
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