Technote | EGF (Qk066)
Qkine mouse epidermal growth factor (EGF) is as biologically active as a comparable alternative supplier protein
Epidermal growth factor (EGF) is involved in the regulation of cell growth and proliferation through the tyrosine kinase receptor EGFR. EGF is an essential growth factor for stimulating the proliferation of induced pluripotent stem cells (iPSC) and embryonic stem cells (ESC) and their subsequent differentiation. EGF is also a key component of many media for developing and maintaining organoids.
Qkine mouse EGF (Qk066) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.
Qkine EGF bioactivity
- Qkine mouse EGF was bioactive in a quantitative luciferase assay with an EC50 of 196.7 pg/ml.
- This was equivalent to mouse EGF from an alternative supplier, which also had an EC50 of 164.9 pg/ml.
Qkine EGF was as bioactive as EGF from an alternative major supplier. Bioactivity was determined using the Promega serum response element (SRE) luciferase reporter assay in transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of Qkine EGF (Qk066, green) or Peprotech EGF (Supplier B, black) for 3 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.
The bioactivity comparison demonstrates that Qkine EGF has equivalent bioactivity to EGF from an alternative major supplier. Qkine proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
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