Technote | LIF (Qk036)
Qkine leukemia inhibitory factor (LIF) is as biologically active as a comparable alternative supplier protein
Leukemia inhibitory factor (LIF) belongs to the IL-6 cytokine family and regulates embryonic development, immune response, and inflammation. LIF is essential to maintain stem cell pluripotency, so it is widely used in embryonic and induced pluripotent stem cell cultures. LIF can also differentiate and maintain immune cells, such as T cells and macrophages.
Qkine LIF (Qk036) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.
Qkine LIF bioactivity
- Qkine LIF (Qk036) stimulated the HEK293T luciferase reporter assay with an EC50 of 0.28 ng/ml (16 pM).
- This was comparable to Supplier B LIF bioactivity of 0.29 ng/ml (17 pM).
Qkine LIF was as bioactive as LIF from an alternative major supplier. Bioactivity was determined using the Promega serum response element luciferase reporter assay in transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of Qkine LIF (Qk036, green) or Peprotech LIF (300-05, Supplier B, black) for 24 hours. Protein concentrations were measured before use (Peprotech vial recovery 101%). Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.
The bioactivity comparison demonstrates that Qkine LIF has equivalent bioactivity to LIF from an alternative major supplier. Qkine LIF (Qk036) has the advantage of being highly pure and animal origin-free, giving batch-to-batch consistency in bioactivity for long-term reproducible culture of stem and immune cells.
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