Technote | HGF (Qk013)
Qkine hepatocyte growth factor (HGF) is bioactive up to 7 days in cell culture conditions
Hepatocyte growth factor (HGF) is a multi-functional cytokine that promotes the growth and proliferation of various cell types including mesenchymal, endothelial, and epithelial cells. It is involved in embryonic development, cell motility, survival, angiogenesis, and tissue regeneration. In cell culture, HGF is used to promote the differentiation of mesenchymal cells towards hepatocytes, osteocytes, and endothelial cells.
Qkine Recombinant human HGF NK1 (Qk013) protein is a potent naturally occurring isoform of HGF. This protein is animal origin-free, carrier protein-free, and tag-free to ensure its purity with exceptional lot-to-lot consistency.
Qkine HGF NK1 stability
- Some slight degradation of HGF NK1 protein could be seen in an SDS-PAGE gel over 7 days.
- This was reflected in a slight decrease in bioactivity, with an EC50 at day 0 of 0.9 ng/ml, day 2 EC50 1.4 ng/ml and day 7 EC50 3.5 ng/ml.
Stability and bioactivity of Qkine HGF NK1. SDS-PAGE of recombinant HGF NK1 (A), protein was partially degraded when incubated in HEK293T conditioned media for 7 days. Bioactivity was determined using the HGF NK1-responsive firefly luciferase reporter assay (B). Transfected HEK293T cells were treated in triplicate for 6 hours with a serial dilution of HGF NK1 which had been pre-incubated in conditioned media for 2 or 7 days. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity.
Qkine HGF NK1 was bioactive under cell culture conditions for up to 7 days. Qkine proteins highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture and differentiation of human pluripotent stem cells.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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