Technote | HGF ()
High purity, animal and carrier protein-free HGF protein for chemically-defined media and more reproducible scale-up
Hepatocyte growth factor (HGF) is a multi-functional cytokine that promotes the growth and proliferation of various cell types including mesenchymal, endothelial, and epithelial cells. It is involved in embryonic development, cell motility, survival, angiogenesis, and tissue regeneration. In cell culture, HGF is used to promote the differentiation of mesenchymal cells towards hepatocytes, osteocytes, and endothelial cells.
Qkine Recombinant human HGF NK1 (Qk013) protein is a potent naturally occurring isoform of HGF. This protein is animal origin-free, carrier protein-free, and tag-free to ensure its purity with exceptional lot-to-lot consistency.
Qkine HGF NK1 differentiation of iPSC to hepatocyte-like cells
Recombinant human HGF NK1 protein promotes efficient differentiation of human iPSCs to hepatocyte-like cells. HGF NK1 supports human iPSC differentiation to hepatocyte-like cells. Defined media was supplemented with HGF NK1 (Qk013) during day 8-18 of the hepatocyte differentiation protocol (adapted from Heck W, et al. 2015). Cells attain characteristic hexagonal epithelial morphology. Expression of hepatic marker, HNF4α, and polarization marker, E-Cadherin (ECAD), was detected using immunofluorescence.
Highly homogeneous expression of HNF4α in hepatocyte-like cells differentiated from iPSCs using Qk013 HGF NK1. Growth in media supplemented with HGF NK1 (Qk013) leads to highly homogeneous expression of hepatic marker, HNF4α. iPSCs were treated with hepatocyte differentiation media supplemented with HGF NK1 or full-length, mammalian cell-derived HGF from another commercial source (supplier x).
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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