Technote | GDF-5 (Qk070)
Qkine human growth differentiation factor 5 (GDF-5) bioactivity is comparable to an alternative supplier protein
Recombinant human GDF-5 protein is commonly used for the differentiation and maintenance of induced pluripotent stem cells, embryonic stem cells, or bone marrow-derived mesenchymal stem cells into osteoblasts and chondrocytes.
Qkine GDF-5 (Qk070) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.
Qkine GDF-5 bioactivity
- GDF-5 (Qk070) was found to be bioactive in a quantitative luciferase reporter assay, with an EC50 of 1.1 ng/ml.
- This was comparable to GDF-5 from an alternative supplier, which had an EC50 of 1.0 ng/ml.
Qkine GDF-5 was as bioactive as GDF-5 from an alternative major supplier. Bioactivity was determined using a GDF-5-responsive firefly luciferase reporter assay. Stably transfected HEK293T cells were treated in triplicate with a serial dilution of Qkine GDF-5 (Qk070, green) or Peprotech (120-01, Supplier B, black) for 24 hours. Protein concentrations were measured before use (Peprotech vial recovery 108%, Qkine 107%). Firefly luciferase activity was measured and normalised to the control Renilla luciferase activity. Data from Qk070 lot #204559.
Qkine recombinant proteins are highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
About our products
Nine-point Quality Commitment
Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility.
Quality policy
Learn more about our quality management system implemented to comply with the requirements of ISO 9001: 2015.
Animal-free
Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
Contact us
Our science team is here to help, please contact us if you have any questions.
