Technote | G-CSF (Qk074)
Qkine granulocyte colony-stimulating factor (G-CSF) is as biologically active as a comparable alternative supplier protein
Granulocyte colony-stimulating factor (G-CSF) is a member of the hematopoietic growth factor family which plays a crucial role in the proliferation, differentiation, and maturation of committed progenitor cells to granulocytes, such as neutrophils.
Qkine human G-CSF is carrier-free, tag-free, and non-glycosylated to ensure a pure and homogenous production of high-quality neutrophils and other relevant cell cultures with exceptional lot-to-lot consistency.
Qkine G-CSF bioactivity
- Qkine G-CSF (Qk074) was bioactive in a quantitative assay with an EC50 of 48 pg/ml.
- This was comparable to bioactivity of G-CSF from an alternative supplier, which had an EC50 of 45 pg/ml.
Qkine G-CSF was as biologically active as a comparable supplier protein. Recombinant G-CSF activity was determined using the Promega CellTiter-Glo luminescent cell viability assay. NFS-60 mouse myeloid leukemia cells were treated in triplicate with a serial dilution of Qkine G-CSF (Qk074, green) or Peprotech G-CSF (Supplier B, black) for 48 hours. Cell viability was measured and normalized. Data from Qk074 lot #204487.
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of hematopoietic stem cells.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
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