Technote | Flt3L (Qk087-CTGQk087)
Lyophilized Flt3L remains bioactive, stable and sterile after > 2 years storage in the freezer
Fms-like tyrosine kinase 3 ligand (Flt-3 Ligand or Flt3L) is a cytokine that is involved in the regulation of hematopoiesis. It stimulates the survival, proliferation, and differentiation of various early myeloid and lymphoid progenitor cells. Flt3L is commonly used in the differentiation of hematopoietic stem cells into dendritic cells.
Qkine Flt3L (Qk087) is animal origin-free, carrier protein-free, non-glycosylated and tag-free to ensure high and consistent bioactivity.
Qkine Flt3L > 2 years stability
- Flt3L stored at -80 °C for > 2 years (EC50 0.78 ng/ml) showed no difference in bioactivity compared to the newest available batch (EC50 0.94 ng/ml).
- SDS-PAGE showed no degradation of Flt3L was visible after > 2 years.
- Sterility tested showed no contamination after storage at -80 °C for > 2 years.
Flt3L remained stable and bioactive after > 2 years storage in the freezer. (A) Bioactivity was determined using a Flt3L responsive firefly luciferase reporter assay; transfected HEK293T cells were treated in triplicate with a serial dilution of Flt3L (Qk087) stored at -80 °C for > 2 years (green, > 2 years) or from the newest available batch (black, Control). Firefly luciferase activity was measured and normalized to control Renilla luciferase activity. (B) Lyophilized protein was stored at -80 °C for > 2 years. 3 µg was resolved on a 15% w/v SDS-PAGE gel under non-reduced conditions and stained with Coomassie Brilliant Blue R250. Data from Qk087 #204566 (test) and #204633 (control).
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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