Technote | FGF-2 (Qk025-CTGQk025)
Lyophilized FGF-2 145 aa (Qk025) remains bioactive, stable and sterile after > 2 years storage in the freezer
Human fibroblast growth factor 2 (FGF-2/bFGF) protein (145 aa) is our gold-standard and recommended FGF-2 / bFGF protein for induced pluripotent stem cell (iPSC) and embryonic stem cell (ESC) maintenance, and iPSC and mesenchymal stem cell (MSC) proliferation and differentiation. The 145 aa version comprises the core structured region sufficient for full biological activity.
Qkine human FGF-2 (145 aa) protein (Qk025) is highly bioactive, animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.
Qkine FGF-2 (145 aa) > 2 years stability
- FGF-2 (145 aa) stored at -80 °C for > 2 years (EC50 0.12 ng/ml) showed no difference in bioactivity compared to the newest available batch (EC50 0.19 ng/ml).
- SDS-PAGE showed no degradation of FGF-2 was visible after > 2 years.
- Sterility tested showed no contamination after storage at -80 °C for > 2 years.
FGF-2 remained stable and bioactive after > 2 years storage in the freezer. (A) Bioactivity was determined using the Promega serum response element luciferase reporter assay in transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of FGF-2 145 aa (Qk025) stored at -80 °C for > 2 years (green, > 2 years) or from the newest available batch (black, Control). Firefly luciferase activity was measured and normalized to control Renilla luciferase activity. (B) Lyophilized protein was stored at -80 °C for > 2 years. 3 µg was resolved on a 15% w/v SDS-PAGE gel under non-reduced conditions and stained with Coomassie Brilliant Blue R250. Data from Qk025 lot #204544 (test) and #204706 (control).
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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