Technote | FGF-18 (Qk069)
Qkine human fibroblast growth factor-18 (FGF-18) is as biologically active as a comparable alternative supplier protein
Fibroblast Growth Factor 18 (FGF-18), a member of the FGF family, characterized by its heparin-binding properties plays a significant role in regulating diverse biological processes such as embryonic development, skeletal and bone development, cartilage maintenance, angiogenesis and tissue repair.
FGF-18 is widely used to support cell culture maintenance and proliferation, promote chondrogenic and osteogenic differentiation of stem cells and stimulate angiogenesis.
Qkine FGF-18 (Qk069) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.
Qkine FGF-18 bioactivity
- Qkine FGF-18 (Qk069) was bioactive in a quantitative luciferase assay with EC50 of 8.76 ng/ml (0.43 nM).
- This was comparable to FGF-18 from an alternative supplier, which also had an EC50 of 9.45 pg/ml (0.47 nM).
Qkine FGF-18 was found to be as bioactive as FGF-18 from an alternative major supplier. Bioactivity was determined using a FGF-18-response luciferase reporter assay in HEK293T cells. Cells were treated in triplicate with a serial dilution of Qkine FGF-18 (Qk069, green) or Peprotech (100-28, Supplier B, black) for 3 hours. Protein concentrations were measured before use (Peprotech recovery 94%, Qkine 101%). Firefly luciferase activity is measured and normalized to control Renilla luciferase activity.
The bioactivity comparison demonstrates that Qkine FGF-18 has equivalent bioactivity to FGF-18 from an alternative major supplier. Qkine proteins have the advantage of being highly pure and animal origin-free, giving batch-to-batch consistency in bioactivity for long-term reproducible culture and differentiation of stem cells.
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