Technote | BDNF (Qk050)
Qkine BDNF was as bioactive as the WHO (NIBSC) standard BDNF
Brain-derived neurotrophic factor (BDNF) is a member of neurotrophin family and plays a crucial role in neural development, maintenance, and function. It stimulates neurogenesis and is also a major regulator of synaptic plasticity and neuroprotection. BDNF is essential for neuronal maintenance and differentiation for neural regenerative stem cell therapies.
Cell therapy grade BDNF protein has a molecular weight of 14 kDa, animal origin-free, carrier-free, tag-free, and non-glycosylated to ensure its purity with exceptional lot-to-lot consistency.
Qkine BDNF bioactivity
- Qkine BDNF was bioactive in a quantitative luciferase assay with an EC50 of 1.46 ng/ml (104 pM).
- This was as bioactivity as the WHO (NIBSC) standard BDNF (NIBSC code: 96/534), which had an EC50 of 1.36 ng/ml.
Qkine BDNF was as bioactive as the WHO (NIBSC) standard BDNF. BDNF activity was determined using a NFAT luciferase reporter assay in transiently transfected HEK293 cells also expressing TrkB. Transfected cells were treated in triplicate with a serial dilution of BDNF for 6 hours. Firefly activity was measured and normalized to the control Renilla luciferase activity. Data from Qk050 #204714.
Qkine cell therapy grade high purity animal origin-free proteins are manufactured as GMP grade equivalents in an ISO 9001:2015-certified facility, under ISO 20399:2022 standards with GMP compliance, defined quality criteria and documentation.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility.
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Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an ISO 9001: 2015 certified facility.
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