Technote | Activin A (Qk001-CTGQk001)
Lyophilized activin A remains bioactive, stable and sterile after > 2 years storage in the freezer
Activin A is frequently used to maintain pluripotency in induced pluripotent and embryonic stem cell cultures. It is also used in many stem cell differentiation protocols, including endoderm lineage differentiation and further maturation into hepatocyte and pancreatic cells.
Qkine activin A (Qk001) is a high-purity mature bioactive dimer which is animal origin-free, carrier protein-free and tag-free.
Qkine activin A > 2 years stability
- Activin A stored at -80 °C for > 2 years (EC50 5.1 pM) showed no difference in bioactivity compared to the newest available batch (EC50 7.8 pM).
- SDS-PAGE showed no degradation of activin A was visible after > 2 years.
- Sterility tested showed no contamination after storage at -80 °C for > 2 years.
Activin A remained stable and bioactive after > 2 years storage in the freezer. (A) Bioactivity was determined using a CAGA reporter luciferase assay; transfected HEK293T cells were treated in triplicate with a serial dilution of activin A (Qk001) stored at -80 °C for > 2 years (green, > 2 years) or from the newest available batch (black, Control). Firefly luciferase activity was measured and normalized to control Renilla luciferase activity. (B) Lyophilized protein was stored at -80 °C for > 2 years. 3 µg was resolved on a 15% w/v SDS-PAGE gel under non-reduced conditions and stained with Coomassie Brilliant Blue R250. Data from Qk001 lot #204545 (test) and #204771 (control).
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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