Technote | Activin A (Qk001Qk001-CTG)
Qkine activin A has consistently high bioactivity lot-to-lot
Activin A is frequently used to maintain pluripotency in induced pluripotent and embryonic stem cell cultures. It is also used in many stem cell differentiation protocols, including endoderm lineage differentiation and further maturation into hepatocyte and pancreatic cells.
Qkine activin A (Qk001) is a high-purity mature bioactive dimer which is animal origin-free, carrier protein-free and tag-free.
Qkine activin A lot-to-lot bioactivity
- The bioactivity of independent lots of Qkine activin A (Qk001) were tested in a quantitative luciferase assay.
- Each lot had consistent bioactivity in the range 0.26-0.29 ng/ml. Lot #204536 EC50 = 0.26 ng/ml, lot #204545 EC50 = 0.29 ng/ml, lot #204564 EC50 = 0.27 ng/ml.
Qkine activin A had consistent bioactivity over 3 independently manufactured lots. Activin A activity was determined using the activin-responsive firefly luciferase reporter assay in transiently transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of 3 independent lots of Qkine activin A. Firefly luciferase activity was measured and normalized.
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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