Technote | Activin A (Qk001-CTGQk001)
Qkine activin A was more active than the WHO (NIBSC) standard activin A
Activin A is a TGF-β family growth factor frequently used to maintain pluripotency in induced pluripotent and embryonic stem cell cultures. It is essential for many stem cell differentiation protocols, including endoderm lineage differentiation and further maturation into hepatocyte and pancreatic cells for regenerative cell therapy applications.
Qkine cell therapy grade activin A (Qk001-CTG) is a high-purity mature bioactive dimer which is animal origin-free, carrier protein-free and tag-free.
Qkine activin A bioactivity
- Qkine activin A was bioactive in a quantitative luciferase assay with an EC50 of 0.27 ng/ml (10.4 pM).
- This was higher than the bioactivity of the WHO (NIBSC) standard activin A (NIBSC code: 91/626), which had an EC50 of 0.66 ng/ml.
Qkine activin A was more active than the WHO (NIBSC) standard activin A. Activin A activity was determined using a CAGA luciferase reporter assay in transiently transfected HEK293 cells. Transfected cells were treated in triplicate with a serial dilution of activin A for 6 hours. Firefly activity was measured and normalized to the control Renilla luciferase activity. Data from Qk001 lot #204564.
Qkine cell therapy grade high purity animal origin-free proteins are manufactured as GMP grade equivalents in an ISO 9001:2015-certified facility, under ISO 20399:2022 standards with GMP compliance, defined quality criteria and documentation.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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