StemBeads® Qkine BDNF£290.00
StemBeads® Qkine BDNF
£290.00
StemBeads® Qkine BDNF is a patented growth factor supplement that offers a novel way to culture cells with native BDNF more efficiently, with greater control and fewer medium changes. StemBeads® Qkine BDNF has been tested on neural progenitor cell (NPC) media, including neural progenitor expansion medium (NPEM), with enhanced cellular profiles.
StemBeads® revolutionary controlled-release protein technology combined with Qkine high purity and bioactivity animal origin-free growth factors and cytokines stabilizes growth factor levels for increased cell culture consistency and reproducibility.
In stock
Orders are typically shipped same or next day (except Friday).
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£290.00
1000 µg will be dispatched as 2 x 500 µg.
Buy online with secure credit card or purchase order. For any questions, please email ryan.weber@matriqx.com
Summary:
- High purity human BDNF protein (Uniprot: P23560)
Micro-encapsulated high purity protein
StemBeads® technology for controlled-release
Lot-to-lot consistency in bioactivity
- Product supplied in liquid form
Featured applications:
Differentiation of midbrain dopaminergic neurons
Differentiation of iPSC derived neural progenitors to neurons
Neural organoid growth and differentiation
Neural differentiation from pluripotent stem cells
Frequently used together
Alternative proteins
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
StemBeads® Qkine BDNF is a patented growth factor supplement that offers a novel way to culture cells with native BDNF more efficiently, with greater control and fewer medium changes. BDNF is key for supporting neural progenitor cells (NPCs) and neuronal differentiation. StemBeads® Qkine BDNF are microbeads composed of an FDA approved, biodegradable PLGA polymer that is loaded with Qkine recombinant human BDNF (Qk050) and release the encapsulated protein at a constant rate. Controlled delivery and stable levels overcome the half-life of BDNF and improve cell cultures while saving researchers valuable time and resources.
StemBeads are a revolutionary controlled-release growth factor technology. The StemBeads product is currently used for improved cell culture in more than 100 scientific laboratories globally. StemBeads technology encapsulates a growth factor in a PLGA hydrogel that stabilizes the protein and releases it steadily over several days. With growth factor levels stabilized, spontaneous differentiation is reduced, pluripotency is increased, and feeding schedules are reduced to only 2-3 times per week. StemBeads are added to your medium to achieve a desired growth factor release, sustained over several days. Following, the beads are washed from the culture, the medium is replaced, and the process repeated as required.
Brain-derived neurotrophic factor (BDNF) is a member of neurotrophin family which includes nerve growth factor (NGF) and neurotrophin–3/4/5 (NT–3/4/5) [1,2]. BDNF plays a crucial role during embryonic development and the maintenance of the nervous system during adulthood. It mediates many aspects, including the survival, growth, maintenance, and differentiation of neural stem cells and mature neurons [3,4]. It stimulates neurogenesis and promotes the growth of dendrites and axons [5,6]. It is also involved in synaptic plasticity, forming and strengthening synapses in response to experience and learning [7]. Finally, it regulates the neuroprotection as it is involved in neural repair and recovery [8].
In cell culture, recombinant human BDNF supports the survival and growth of neurons and establishes and strengthens synapses. It is used in combination with other growth factors to maintain neurons and to differentiate human-induced pluripotent stem cell-derived neural progenitors into neurons [5]. It is used for the differentiation and maturation of dopaminergic neurons with glial-derived neurotrophic factor (GDNF), FGF-8a/FGF-8b, and Shh [9–11]. Glutamatergic and GABAergic neurons are maintained with combinations of BDNF, GDNF, NT-3, or insulin-like growth factor 1 (IGF-1) [12–14]. Cholinergic neurons are obtained using BDNF, IGF-1, and NGF [15,16]. Also, the culture of cortical organoids require BDNF and GDNF for maturation [17].
Additional resources
FAQ
Will StemBeads® interfere with my downstream application of the cells?
StemBeads® can be easily removed prior to analysis by washing 1-2 times with PBS, DMEM, F12 or any other basal medium preferred. For suspension cultures, there are a variety of strainer pore sizes to allow for separation. Cells cultured with StemBeads® have been used in a variety of downstream applications, including but not limited to: immunofluorescence, FACS, RNA/DNA extraction, nucleofection, differentiation etc.
What if I want to use a different concentration, can the protein release be adjusted?
The protein concentration can be adjusted based on the number of StemBeads® that you use. The release rate has a linear correlation with the amount of StemBeads® used. For example, 8 µl of StemBeads® per ml of media releases at a concentration of 10 ng/ml. However, if you desire half of that concentration, such as 5 ng/ml, then adjust the concentration to 4 µl of StemBeads® per ml media to achieve the concentration of 5 ng/ml.
How often do I change media when using StemBeads®?
With growth factor levels stabilized, spontaneous differentiation is reduced, pluripotency is increased, and feeding schedules are reduced to only 2-3 times per week. StemBeads are added to your medium to achieve a desired growth factor release, sustained over several days. Following, the beads are washed from the culture, the medium is replaced, and the process repeated as required.
The pH indicator in my growth medium appears very yellow when not changing media daily. Is this a problem?
No, this is not a problem or indication of cell quality. Extensive testing has been performed to access the cell quality when using StemBeads®. All phenotypic and functional assays have shown cell quality as good or better than current feeding regimens. For best assessment of cells, wash cells once prior to observation.
What does BDNF do?
BDNF plays a crucial role during embryonic development and the maintenance of the nervous system during adulthood including the survival, growth, maintenance, and differentiation of neurons, growth of dendrites and axons, and synaptic plasticity.
What is the BDNF gene?
The BDNF protein is encoded by the BDNF gene on chromosome 11.
What is the difference between NGF, GDNF and BDNF?
NGF, GDNF, and BDNF are all part of the neurotrophins or neurotrophic factors family. They all regulate the development, growth, and survival of neurons and have more specific roles. NGF is associated with the growth and maintenance of sensory and sympathetic neurons. BDNF and GDNF are both associated with dopaminergic, glutamatergic, and GABAergic neurons. In addition, BDNF plays a crucial role in synapses formation whereas GDNF is involved in the growth and maintenance of other glial cells such as astrocytes.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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