Recombinant human KGF (FGF-7) protein (Qk046-CTG) Price range: £3,795.00 through £5,940.00View product
Recombinant human KGF (FGF-7) protein (Qk046-CTG)
Price range: £3,795.00 through £5,940.00
Human KGF protein (FGF-7) is a member of the FGF family. Recombinant KGF protein is used in the culture of lung, breast, liver, and bladder organoids.
Qkine human KGF (FGF-7) is a highly pure and bioactive 19 kDa protein, animal-origin free (AOF) and carrier-protein free (CF).
Qkine cell therapy grade high purity animal origin-free proteins are manufactured as GMP grade equivalents in an ISO 9001:2015-certified facility, under ISO 20399:2022 standards with GMP compliance, defined quality criteria and documentation.
This product is available to order now and will be in stock soon, please contact us for the estimated lead time ryan.weber@matriqx.com
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £3,795.00 through £5,940.00
1000 µg will be dispatched as 2 x 500 µg.
Buy online with secure credit card or purchase order. For any questions, please email ryan.weber@matriqx.com
Summary:
- High purity KGF (FGF-7) protein (Uniprot: P21781)
- 19 kDa
Certified animal origin-free (AOF)
Cell and gene therapy grade
Manufactured in our ISO 9001:2015 facility in Cambridge, UK
Lot-to-lot consistency in bioactivity
Full traceability and documentation
Carrier protein-free, tag free
Expressed in E. coli
Lyophilized from PBS
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Regenerative stem cell therapy
Alternative proteins
KGF (FGF-7) activity was determined using the Promega serum response element luciferase reporter assay in transfected MCF-7 cells. Cells were treated in triplicate with a serial dilution of KGF for 4 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. EC50 = 1.75 ng/ml (92 pM). Data from Qk046 lot #104314.
KGF migrates as a monomer at 19 kDa and a dimer at 38 kDa in non-reducing (NR) conditions and 19 kDa upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk046 lot #104314.
Further quality assays
Mass spectrometry: single species with expected mass
Purity >98%, by SDS-PAGE quantitative densitometry
Recovery from stock vial: >95%
Sterility tested and mycoplasma negative
Endotoxin: <0.05 EU/μg protein
Residual host cell protein <10 ng/µg
Residual host cell DNA <10 ng/μg
N-terminal sequence analysis
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine KGF (FGF-7) was more active than the WHO (NIBSC) standard KGF. KGF (FGF-7) activity was determined using the Promega serum response element luciferase reporter assay in transfected MCF-7 cells. Cells were treated in triplicate with a serial dilution of KGF for 4 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Qk046 204737 EC50 = 0.70 ng/ml. WHO (NIBSC) EC50 = 1.43 ng/ml.
Protein background
Keratinocyte growth factor (KGF), also known as fibroblast growth factor-7 (FGF-7), is part of the large FGF family, and, alongside FGF-3, FGF-10, and FGF-22, is a member of the FGF-7 subfamily. This subfamily is secreted exclusively by the mesenchyme, and specifically activates the “b” isoforms of FGFR1 (FGFR1b) and FGFR2 (FGFR2b) present in the overlying epithelium [1], of these receptors KGF acts through the IIIb splice form of FGFR2. KGF is required for the development of the kidney, thymus, and hippocampus [2]. KGF also plays an import role in wound healing [3], a role exemplified in studies on bioengineered skin [3]. KGF protein has been used in generation of many organoids including lung, breast, liver and bladder [2].
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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