Recombinant human IGF-2 protein (Qk118) Price range: £175.00 through £880.00View product
Recombinant human IGF-2 protein (Qk118)
Price range: £175.00 through £880.00
Insulin-like Growth Factor 2 (IGF-2) is a growth factor that promotes cell proliferation, differentiation, and survival, particularly during fetal development. IGF-2 also regulates tissue growth and muscle regeneration. Recombinant IGF-2 stimulates proliferation and differentiation in various stem cell cultures.
Qkine human IGF-2 is a highly pure 7.5 kDa monomer, animal origin-free (AOF) and carrier-protein-free (CF). Qkine has optimized the IGF-2 manufacturing process to produce a highly bioactive protein with excellent lot-to-lot consistency for enhanced experimental reproducibility.
This protein is also available as GMP compliant Cell Therapy Grade, to enquire email ryan.weber@matriqx.com.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £175.00 through £880.00
1000 µg will be dispatched as 2 x 500 µg.
Buy online with secure credit card or purchase order. For any questions, please email ryan.weber@matriqx.com
Summary:
- High purity human IGF-2 protein (Uniprot: P01344)
- 7.5 kDa
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Cellular proliferation, migration and survival
iPSC and primary cell differentiation to muscle
Osteogenic differentiation
Recombinant IGF-2 activity was determined using an SRE reporter assay on transfected MCF-7 cells. Cells were treated in triplicate with a serial dilution of IGF-2 for 4 hours. Firefly activity was measured and normalized to the control Renilla luciferase activity. Data from Qk118 lot 204687. EC50 = 58 ng/ml (7.8 nM)
Recombinant IGF-2 migrates at approximately 7.5 kDa in reduced (R) and non-reduced (NR) conditions. No contaminating protein bands are present. The purified recombinant protein (3 µg) was resolved using 18% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk118 lot #204687.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial: >95%
Endotoxin: <0.05 EU/μg protein
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine IGF-2 is as biologically active as the comparable alternative supplier protein. IGF-2 activity was determined using the serum response element (SRE) firefly luciferase reporter assay in transfected MCF-7 cells. Cells were treated in triplicate with a serial dilution of IGF-2 (Qk118, green) or alternative supplier IGF-2 (supplier B, black). Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk118 lot #204687.
Protein background
IGF-2, or Insulin-like Growth Factor 2, is a protein hormone that plays a crucial role in growth and development, particularly during fetal development. It is part of the insulin-like growth factor family, which includes IGF-1 and insulin. IGF-2 is a single-chain polypeptide consisting of 67 amino acids, with a molecular weight of 7.5 kDa. The protein’s three-dimensional structure is characterized by three alpha-helices stabilized by three disulfide bonds [1]. These disulfide bonds are formed between six conserved cysteine residues, which are critical for maintaining the protein’s tertiary structure and biological activity. The overall fold of IGF-2 is similar to that of insulin, reflecting their evolutionary relationship [2].
IGF-2 has numerous applications across various fields. In prenatal development, it is crucial for fetal growth and development, influencing organ size and body composition. It’s used as a marker in prenatal diagnostics to assess fetal growth and potential developmental issues [3]. In cancer research, IGF-2 has been implicated in various cancers, including colorectal, breast, and prostate cancer. It’s being studied as a potential biomarker for early cancer detection and as a target for cancer therapies. Given its role in glucose metabolism, IGF-2 is also being investigated in the context of metabolic disorders such as diabetes and obesity, with researchers exploring its potential in developing new treatments [4].
IGF-2 has tissue engineering and regenerative medicine applications, particularly in wound healing and tissue repair. IGF-2 has neuroprotective effects, suggesting potential applications in treating neurodegenerative disorders like Alzheimer’s disease. IGF-2 gene polymorphisms are used as genetic markers for selective breeding to improve meat quality and production efficiency [5]. Additionally, as a growth factor, IGF-2 is monitored in sports medicine to detect potential abuse for performance enhancement. The ongoing research into IGF-2’s structure and functions continues to open new avenues for applications in medicine, biotechnology, and agriculture, underlining its significance across multiple disciplines [6].
Additional resources
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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